A highthroughput colorimetric assay for detection of schistosoma. The aim of this study was to improve xtt reduction assay by adding. Xtt 2,3bis2methoxy4nitro5sulfophenyl2htetrazolium5carboxanilide is a tetrazoliumbased compound like the reagents used in the mtt assay and the mts assay. Thus, xtt can be used to assess cell proliferation or cytotoxicity of drugs. The colorimetric reduction of xtt by cellular enzymes xtt cell proliferation assay kit instruction manual catalog number 301011k assays store at 20oc this product is intended for laboratory research purposes only. Xtt reagent is used to assess cell viability as a function of cellular redox.
The cyquant xtt cell viability assay is a complete, optimized assay that generates a consistent colorimetric detection of viable mammalian cells. Review our cell health assay guide to learn about our kits to perform a cell viability assay, cytotoxicity assay or cell proliferation assay. Unlike mtt, xtt does not require solubilization prior to quantitation, thereby reducing the assay time in many viability assay protocols. The procedure of xtt is simply for measuring proliferation and is therefore an excellent solution for. The assay kit consists of two reagents, xtt reagent and electron coupling reagent. Prepare xtt working solution by combining xtt reagent with xtt activator according to the above. Culture the cells for 2496 hours at 37c and 5% co 2. Results xtt assay figure 1 xtt assay for fibroblasts beas. March 2016 1 kit for 2,500 tests store at 15 to 25c 1. Cellular viability xtt assay protocol this assay is based on the conversion of the watersoluble xtt 2,3bis2methoxy4nitro5sulfophenyl2htetrazolium5carboxanilide reagent to an orange formazan product by actively respiring cells.
The xtt assay has been reported to be more sensitive than the mtt. Xtt assay is a colorimetric method that uses the tetrazolium dye. A collection of mtt assay protocols for research, provided by invitrogen. This xtt viability assay was validated for high throughput screening of. Pdf improvement of xtt assay performance for studies involving.
In this chapter the protocol of the assay is described including important considerations relevant for each. Download kit booklet pdfdownload safety data sheet sds pdf. Cell proliferation kit ii xtt colorimetric assay xtt based for the nonradioactive quantification of cell proliferation and viability. Xtt reagent is used to assess cell viability as a function of cellular.
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